Isotope dilution analysis is a method of determining the number of chemical substances. In its most simple conception, the isotope dilution method comprises known amounts of an isotopically-enriched substance to the analyzed sample. Mixing the isotopic standard with the sample effectively "dilutes" the standard's isotopic enrichment, which forms the basis for the isotope dilution method. Thus, isotope dilution is classified as a method of internal standardization because the standard (an isotopically-enriched form of the analyte) is added directly to the sample.